Please be advised of the following prior to use sodium azide for storage : But please be advised that any serum, like Test Sera for animal selection, pre-immune sera (PPI), large bleed sera (GP), or final bleed sera (SAB) do not contain any preservative. Purified custom-made antibodies from Eurogentec already contain 0,01% thimerosal to avoid microbial growth, as mentioned on the data sheet. Minimisation of aggregate formation of some IgG isotypes (IgG3 for instance) requires storage at 4☌.Storage at –80☌ of glycerol containing solutions is not advised since this is below the freezing point of glycerol PBS), and preservatives (azide or thimerosal), furthermore, please be sure to use a sterile glycerol formulation, since microbial growth might occur and destroy the antibody. Fluorophore-conjugated antibodies can be stored aliquoted at –20☌ with 50% glycerol (final v/v) – please make sure by the data sheet that they are formulated as well with BSA, physiologically concentrated buffer salts (e.g.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |